Description
Principle of the assay: The kit uses a double-antigen sandwich enzyme-linked immunosorbent assay (ELISA) to analyze the existence or not of Human aPT1a/PT2 in samples. Add sample to wells pre-coated with one Human Prothrombins antigen, at same time add HRP-conjugated Human Prothrombins antigen to bind the analyte, followed by incubation and washing procedures to remove unbound substance. Finally, HRP substrates are added, incubated for detection, and a blue color is developed. Reaction is stopped and color turns to yellow when Stopping Solution (acidic) is added. The existence or not of Human aPT1a/PT2 in the samples is then determined by comparing the O.D. of the samples to the CUT OFF